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KMID : 0615720100270040223
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Jouranl of Korean Acupuncture and Moxibustion society
2010 Volume.27 No. 4 p.223 ~ p.231
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The Effects of Euonymi Ligunum Suberalatum Phamacopuncture on Nitric Oxide and Interleukin-6 Production in Macrophage
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Jeon Jae-Cheon
Lee Eun-Yong
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Abstract
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Objectives : The objective of this study is to study the effects of Euonymi Lignum Suberatatum pharmacopuncture solution on NO and IL-6 production in macrophage.
Methods : At first, the RAW 264.7 macrophage was subclutured. In order to evaluate cytotoxicity, MTT assay performed. Then, the cell was induced by LPS, INF-γ and Experimental groups were divided into five(Normal, Control, Euonymi Lignum Suberatatum 100, 200, 300㎍/㎖). Then Euonymi Lignum Suberatatum pharmacopuncture solution was put into cell. We measured IL-6, iNOS, NO.
Results : The cytotoxic effect of Euonymi Lignum Suberatatum pharmacopuncture solution in RAW 264.7 macrophage was not appeared. 300㎍/㎖ Euonymi Lignum Suberatatum pharmacopuncture solution inhibited IL-6 production in LPS, INF-γ-stimulated RAW 264.7 macrophages significantly. Euonymi Lignum Suberatatum pharmacopuncture solution inhibited iNOS revelation in LPS, INF-γ- stimulated RAW 264.7 macrophages. All group of Euonymi Lignum Suberatatum pharmacopuncture solution inhibited NO production in LPS, INF-γ-stimulated RAW 264.7 macrophages significantly.
Conclusions : Our study demonstrated that Euonymi Lignum Suberatatum pharmacopuncture solution had an inhibition effect on NO production, iNOS revelation, IL-6 production. So Euonymi Lignum Suberatatum pharmaco puncture solution may have an Anti-inflammation effect.
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KEYWORD
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Euonymi Lignum Suberatatum, Pharmacopuncture, IL-6, iNOS, NO, Anti-inflammation
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